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When exposed to electrophiles (i.e., certain antioxidants) or oxidative stress, Keap1 is inactivated
HAP1 wild type (Horizon #C631, RRID: CVCL_Y019) and HAP1 PDE6D knockout (Horizon #HZGHC006484c003, RRID: CVCL_XR47) cells were purchased from Horizon (Horizon Discovery, UK)
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Glutathione is a tripeptide composed of three amino acids: L-cysteine, L-glutamic acid, and glycine
For proper reconstitution technique across all peptides, consistent gentle mixing is the universal rule
Insert the needle into the AOD 9604 vial at an angle, directing the stream against the glass wall
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